| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114313 |
| Chromosome: | chromosome 17 |
| Location: | 7148031 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g747747 | (1 of 18) PF12906 - RING-variant domain (RINGv) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAATGCCACACNCCTGTCCGTGGGGGTG |
| Internal bar code: | ACCGCGGGTCGACGTTGAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 11 |
| LEAP-Seq percent confirming: | 2.54777 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 153 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGCACGAGATTTCTTCA |
| Suggested primer 2: | GTGATAGCTAGAGGTGCGGC |