| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.114323 |
| Chromosome: | chromosome 3 |
| Location: | 8631580 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203569 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACGCCCCATCCACCGACCACCCTCCCTC |
| Internal bar code: | AGTAACCAGTTCCCTTGAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 543 |
| LEAP-Seq percent confirming: | 98.9154 |
| LEAP-Seq n confirming: | 456 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGAGATGGGGAAAGAGG |
| Suggested primer 2: | GGACGGCATCACTTTTGAGT |