Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.114362 |
Chromosome: | chromosome_6 |
Location: | 2993259 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g273450 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GATGAATGCACGCTGCCGAGCTGGTTGACA |
Internal bar code: | CACCGTGAAGTGGAGAAGGCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 873 |
LEAP-Seq percent confirming: | 99.8497 |
LEAP-Seq n confirming: | 9965 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTATCCGGGAGATGAATGC |
Suggested primer 2: | GGTACTCGGTGGTTTCAGGA |