| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.114362 |
| Chromosome: | chromosome_6 |
| Location: | 2993264 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g273450 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCCCTCCTGCCCTACATGGCCTCCCATAC |
| Internal bar code: | CGGTGATCAAAGTGCGACGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 151 |
| LEAP-Seq percent confirming: | 99.3464 |
| LEAP-Seq n confirming: | 1824 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTATCCGGGAGATGAATGC |
| Suggested primer 2: | GGTACTCGGTGGTTTCAGGA |