| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.114380 |
| Chromosome: | chromosome 12 |
| Location: | 1665664 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486050 | (1 of 1) PF00098//PF00397 - Zinc knuckle (zf-CCHC) // WW domain (WW) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGGCGACCGGTGGACTGATGGGGGCCA |
| Internal bar code: | ACTCTTCAGCGCCTGTCGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1218 |
| LEAP-Seq percent confirming: | 99.715 |
| LEAP-Seq n confirming: | 12246 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTACAGTAACTCCGGCAT |
| Suggested primer 2: | TACCGTACTGGTGATTGGCA |