| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.114399 |
| Chromosome: | chromosome 9 |
| Location: | 775183 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402050 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTGCAGCGGATGAAAGTCACGGTACCA |
| Internal bar code: | CCCACAGCGGCCGGGGTCAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 934 |
| LEAP-Seq percent confirming: | 99.5519 |
| LEAP-Seq n confirming: | 1333 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAAGGGTTGATTTGGAG |
| Suggested primer 2: | CACACAGCCCTGCTACTTGA |