| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.114408 |
| Chromosome: | chromosome 12 |
| Location: | 4883673 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525300 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAGACAGTGCATATCATGTGTTCAATG |
| Internal bar code: | GCAGCTTTATGTGAATGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 99.6748 |
| LEAP-Seq n confirming: | 1839 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCAAGTTCCCTGCATGTC |
| Suggested primer 2: | GGGTTTTCAGCAGGTGTGAT |