| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.114411 |
| Chromosome: | chromosome 2 |
| Location: | 6921016 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119900 | PCP1,CEP6 | (1 of 1) PTHR12411//PTHR12411:SF332 - CYSTEINE PROTEASE FAMILY C1-RELATED // SUBFAMILY NOT NAMED; Cysteine endopeptidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGAGGATGGGCCGGCTGGCCTGGAGGCG |
| Internal bar code: | GAGCCTCGGCATGGGTTTCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 95.5981 |
| LEAP-Seq n confirming: | 3844 |
| LEAP-Seq n nonconfirming: | 177 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCGCTTACACTGTGGCCT |
| Suggested primer 2: | ACTTGTTCCGATGTATCCGC |