Insertion junction: LMJ.RY0402.114444_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.114444
Chromosome:chromosome 16
Location:3663979
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g688600 XPG4 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGCACACATACCCTTTCGGCCAACGACT
Internal bar code:CAAGATCGGCCCGGATCGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:811
LEAP-Seq percent confirming:99.8124
LEAP-Seq n confirming:532
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AACTAGCAACAACGTGCGTG
Suggested primer 2:ACCGAGGTGGAGAAGAAGGT