| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.114448 |
| Chromosome: | chromosome 6 |
| Location: | 2928995 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272701 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTTAATTCCAGCGCGATTTTTGCCTGT |
| Internal bar code: | AGCCCCAGCCTCTGGACTAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 39 |
| LEAP-Seq percent confirming: | 64.4295 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCAGCTATCAAGCACAA |
| Suggested primer 2: | AATCCTCGCAACTACCAACG |