Insertion junction: LMJ.RY0402.114464_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.114464
Chromosome:chromosome 3
Location:1533430
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g152200 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTTCTTGTTCGTGGCTGTTCGTGGCGCC
Internal bar code:TTCAGGTTCGTTTGAGTCGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:60
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTCTTTCTTTTCGCTGTG
Suggested primer 2:TACGGCACTTACTTGGGGAC