| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.114484 |
| Chromosome: | chromosome 9 |
| Location: | 2373984 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391900 | TRXH1,TRXH,TRXh1,Chh1,TRX5 | (1 of 9) K03671 - thioredoxin 1 (trxA); Thioredoxin h1, cytosolic | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAATGCCCACGCTGTGCAGCAACACTGT |
| Internal bar code: | TCGTCGTTAATTATAGGCTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 194 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCTGGCAGATTGTTGTC |
| Suggested primer 2: | CTTCGGCTCCTAAGTTGTCG |