| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114499 |
| Chromosome: | chromosome_2 |
| Location: | 608197 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g077850 | FAP212 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGATGAATGGTGTGGGGTGCGTGATCTGCA |
| Internal bar code: | GATCCCCAGTCGGAACGAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 777 |
| LEAP-Seq percent confirming: | 99.6115 |
| LEAP-Seq n confirming: | 2564 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCAATTGCCAGGTACAGC |
| Suggested primer 2: | TAACGTGACCAAGGACACCA |