| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.114500 |
| Chromosome: | chromosome 14 |
| Location: | 2046804 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621850 | (1 of 4) PF13369 - Transglutaminase-like superfamily (Transglut_core2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATCCGATCCAGGAGCTGTTCCTGCTCCG |
| Internal bar code: | CTCCTGTTTTATGGGAGACTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 473 |
| LEAP-Seq percent confirming: | 98.0392 |
| LEAP-Seq n confirming: | 400 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTTTGTGTGTGTGGGTG |
| Suggested primer 2: | ACGGTACCCAAACAGACCAG |