| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.114505 |
| Chromosome: | chromosome 7 |
| Location: | 3282791 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334350 | (1 of 3) 2.7.1.39 - Homoserine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCACCGCCGCTTGACCTTGCCCTGCTAT |
| Internal bar code: | CGCGTCCAGGGTTCGGAGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 99.2169 |
| LEAP-Seq n confirming: | 20777 |
| LEAP-Seq n nonconfirming: | 164 |
| LEAP-Seq n unique pos: | 100 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCAATATGTAGGCCGTT |
| Suggested primer 2: | GGACCTTCTCGCTCTTGTTG |