Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.114576 |
Chromosome: | chromosome_10 |
Location: | 1708982 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g430350 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAAGACCACATGGGTGCTCTCACCAAGCCA |
Internal bar code: | TTAGCGCATGTTAACGGGACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 822 |
LEAP-Seq percent confirming: | 99.1643 |
LEAP-Seq n confirming: | 1780 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCACCAAACCTTCGATTCAT |
Suggested primer 2: | CGGTGAGTTAGAAGAAGCGG |