| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.114576 |
| Chromosome: | chromosome 10 |
| Location: | 1708983 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430350 | POB23 | Proteome of basal body 23 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTTCCGCCACGGGTTGGGATGGGGCAC |
| Internal bar code: | GAATAACTTAGTATCTATCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 307 |
| LEAP-Seq percent confirming: | 99.5326 |
| LEAP-Seq n confirming: | 2981 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCAAACCTTCGATTCAT |
| Suggested primer 2: | CGGTGAGTTAGAAGAAGCGG |