Insertion junction: LMJ.RY0402.114615_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.114615
Chromosome:chromosome 13
Location:290467
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g564100 PTK2 (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase; Protein tyrosine kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCGAGGTGTGAGGAACATGCGTCCCATA
Internal bar code:CGAAGTCCAGAATCAGTGCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1039
LEAP-Seq percent confirming:99.5297
LEAP-Seq n confirming:2328
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACGAGTCAGCCTTTTAGGCA
Suggested primer 2:GACCTCTTCGGGTTGTGTGT