| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.114623 |
| Chromosome: | chromosome 17 |
| Location: | 1258421 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705350 | CDPKK2 | (1 of 3) K07359 - calcium/calmodulin-dependent protein kinase kinase (CAMKK); Calcium/Calmodulin Dependent Protein Kinase Kinase Homolog 2 isoform beta putative | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGACGGTGACGTGCTCACGCCCCCGTTG |
| Internal bar code: | GGTAGTGGCGTGTAAGAACGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 662 |
| LEAP-Seq percent confirming: | 98.9399 |
| LEAP-Seq n confirming: | 3080 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGCGAAGTTAGAGAAGTG |
| Suggested primer 2: | AAACAGTAACGCACAGCACG |