| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.114669 |
| Chromosome: | chromosome 10 |
| Location: | 1458472 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428664 | WD60,D1bIC1,FAP163,DIC6 | (1 of 2) PTHR12442//PTHR12442:SF26 - DYNEIN INTERMEDIATE CHAIN // WD REPEAT-CONTAINING PROTEIN 34; Dynein 1b Intermediate Chain IC1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCCTCGCCCTTGCACCATCTTGCATGC |
| Internal bar code: | TCCCTCTCAAGCTACTTTATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 99.7547 |
| LEAP-Seq n confirming: | 1220 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTTAGCTGTCGGCATTG |
| Suggested primer 2: | GCACAACTTCATGATCCACG |