| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114670 |
| Chromosome: | chromosome 13 |
| Location: | 4090563 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591400 | COA1,COAA1 | (1 of 1) 2.7.1.33 - Pantothenate kinase / Pantothenic acid kinase; Pantothenate kinase, CoA biosynthesis | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAAGCGGACGGGGGAGAGAAGGGCGGGT |
| Internal bar code: | ACGGAAGGCCGGGGTCAAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 502 |
| LEAP-Seq percent confirming: | 99.1441 |
| LEAP-Seq n confirming: | 4865 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCATGAGTGTATGGGTGC |
| Suggested primer 2: | CGGTTTAGTTTGGGCTGGTA |