| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.114685 |
| Chromosome: | chromosome 12 |
| Location: | 1505900 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487250 | (1 of 1) PF06454 - Protein of unknown function (DUF1084) (DUF1084) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCATGCTCATGTCGGCAAAACATAGCATA |
| Internal bar code: | TCTTATGGTTGCGTGGCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 25 |
| LEAP-Seq percent confirming: | 94.3396 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAGCCAGTTCGACAGAA |
| Suggested primer 2: | CATTTGGTCGACGTTGTGTC |