| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.114697 |
| Chromosome: | chromosome 7 |
| Location: | 96427 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g312800 | (1 of 18) PF12906 - RING-variant domain (RINGv) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGTTGGCGCTTGCCGTGTCAGTATTTC |
| Internal bar code: | TGAGTGACGCACGTTACGCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 99.845 |
| LEAP-Seq n confirming: | 2576 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCATACATTCATGCGAC |
| Suggested primer 2: | GAGTAGCAGGCACCAAGAGG |