| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.114700 |
| Chromosome: | chromosome 13 |
| Location: | 916578 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567901 | (1 of 1) PTHR15451//PTHR15451:SF19 - FAMILY NOT NAMED // ERGOSTEROL BIOSYNTHETIC PROTEIN 28-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCATCACGCCTGGTATCCTCCATGCCCCA |
| Internal bar code: | TGGGCGTTACGTGAAACATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1047 |
| LEAP-Seq percent confirming: | 99.6192 |
| LEAP-Seq n confirming: | 4971 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAGGGACCTCACAACAT |
| Suggested primer 2: | GGTTTTGCCCCAATTCCTAT |