Insertion junction: LMJ.RY0402.114702_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.114702
Chromosome:chromosome 14
Location:3710922
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g631950 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGATGTGGGTCGGGCCGGGATAGCGCTCG
Internal bar code:CGAGTCTCTTTTAGCCTGACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:741
LEAP-Seq percent confirming:61.4025
LEAP-Seq n confirming:1042
LEAP-Seq n nonconfirming:655
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GACACGGATGGACACACAAG
Suggested primer 2:GTTCTATGCCAGCTGCTTCC