| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114702 |
| Chromosome: | chromosome 14 |
| Location: | 3710922 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATGTGGGTCGGGCCGGGATAGCGCTCG |
| Internal bar code: | CGAGTCTCTTTTAGCCTGACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 741 |
| LEAP-Seq percent confirming: | 61.4025 |
| LEAP-Seq n confirming: | 1042 |
| LEAP-Seq n nonconfirming: | 655 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACGGATGGACACACAAG |
| Suggested primer 2: | GTTCTATGCCAGCTGCTTCC |