| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114704 |
| Chromosome: | chromosome 17 |
| Location: | 1098106 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703950 | (1 of 2) 1.1.3.30 - Polyvinyl-alcohol oxidase / PVA oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGGACGCCTTTGGGTGAGAACCTGACT |
| Internal bar code: | GATCGGCCCCGGTTGCAACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 978 |
| LEAP-Seq percent confirming: | 99.7022 |
| LEAP-Seq n confirming: | 4017 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGAAGAACGTCACGGAGC |
| Suggested primer 2: | GACGTTCGTTCTCGGCTAAG |