Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.114714 |
Chromosome: | chromosome_9 |
Location: | 6484700 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g407300 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGATTAGTTAAGGCAGTGGACTGGGGGGT |
Internal bar code: | ATGATCGCGTAAACGTTCTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 401 |
LEAP-Seq percent confirming: | 99.6004 |
LEAP-Seq n confirming: | 8973 |
LEAP-Seq n nonconfirming: | 36 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGAAACCGTGTCTGTGAGT |
Suggested primer 2: | AACCCCTGCAACATCTCATC |