| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.114725 |
| Chromosome: | chromosome 3 |
| Location: | 2892707 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162850 | FAP292 | Flagellar Associated Protein 292 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCACACGTACGCGGTTGTGTGGCCAATC |
| Internal bar code: | ATGCTAAAACGCTCGGACTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 828 |
| LEAP-Seq percent confirming: | 99.4152 |
| LEAP-Seq n confirming: | 850 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCACTTCTTTGCATGACGC |
| Suggested primer 2: | GCGCTGGTGTCATCAGTAGA |