| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.114769 |
| Chromosome: | chromosome 12 |
| Location: | 1683280 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485850 | CPLD64 | Conserved in the Plant Lineage and Diatoms; (1 of 2) PF06916 - Protein of unknown function (DUF1279) (DUF1279) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATTGCATCAGGAGTTGAGGTGTTAAGG |
| Internal bar code: | CTGTCACTCGTATCGGGTAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 98.6547 |
| LEAP-Seq n confirming: | 660 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCAACACAAGAGCGTACC |
| Suggested primer 2: | AAGAAGAAGGAGGAGCCGTC |