| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.114774 |
| Chromosome: | chromosome 2 |
| Location: | 434596 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g076200 | (1 of 1) 3.4.11.2 - Membrane alanyl aminopeptidase / Peptidase E | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTAGCGGCGGCGGCTGTGCAGCGGAGG |
| Internal bar code: | GCGGTCTGCAGTTGGTGCGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 251 |
| LEAP-Seq percent confirming: | 99.9164 |
| LEAP-Seq n confirming: | 1195 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGTTTGGAACGTCAAGG |
| Suggested primer 2: | GACTTTGACACAGCCCGTTT |