| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114783 |
| Chromosome: | chromosome 7 |
| Location: | 4057361 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g340350 | MASAP,P60,ASA1 | Mitochondrial F1F0 ATP synthase associated protein 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTACCGCGCAAGGCAGACGGCTGCTAAG |
| Internal bar code: | TCCGATAACGTGGCGAACGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 390 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGCACATCCAGAAGCAC |
| Suggested primer 2: | TAGCATGCTCATCCCCCTAC |