| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114812 |
| Chromosome: | chromosome 17 |
| Location: | 6634014 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g743797 | FMO8 | Flavin-containing monooxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCTCCCAGCAATGCTCCGCCGCTCCCC |
| Internal bar code: | CATTTTCAGGTCACGGCTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 418 |
| LEAP-Seq percent confirming: | 89.264 |
| LEAP-Seq n confirming: | 1613 |
| LEAP-Seq n nonconfirming: | 194 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCGGTAGACCATCTTGG |
| Suggested primer 2: | GATAGGGATGGAAGTGGCAG |