| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114821 |
| Chromosome: | chromosome 12 |
| Location: | 3981625 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516700 | 3'UTR | ||
| Cre12.g516717 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAGACACTTGAAGAAGCAATTCATGACG |
| Internal bar code: | ACCAGTTTTTGGTCGGCAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 590 |
| LEAP-Seq percent confirming: | 99.5261 |
| LEAP-Seq n confirming: | 2100 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGTGCCTAAGTCTGCGG |
| Suggested primer 2: | ACCGGTGTGGAATATGGTGT |