| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.114858 |
| Chromosome: | chromosome 14 |
| Location: | 3825781 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632700 | (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACCATTCTGATGAACCCGCCCCTCGCT |
| Internal bar code: | CTTAACGTTGCCGGGTTAACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 99.3115 |
| LEAP-Seq n confirming: | 1154 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGCCGTAAATATGAATG |
| Suggested primer 2: | ATGGCTGCACCAGGTAAGTC |