| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.114862 |
| Chromosome: | chromosome 17 |
| Location: | 3463086 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724850 | (1 of 1) K14848 - ribosome assembly protein RRB1 (RRB1, GRWD1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGAGGCGGCGCTGGCGCCCGAGGGCAA |
| Internal bar code: | GGCGGTGGCCGGGACTCCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 729 |
| LEAP-Seq percent confirming: | 99.3593 |
| LEAP-Seq n confirming: | 3412 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAGTAGCATCGTGAGCA |
| Suggested primer 2: | AATGAGGGTGGCAAGTAACG |