| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.114867 |
| Chromosome: | chromosome 3 |
| Location: | 4940621 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179901 | mL116,OPR131,MRPL116 | mitochondrial ribosome protein mL116, Chlamydomonas-specific | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTGTAAGTTCTGGGACATGTAGAGGGC |
| Internal bar code: | TGCGGTTATCACGATGTATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 783 |
| LEAP-Seq percent confirming: | 97.9158 |
| LEAP-Seq n confirming: | 2443 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCGTACATAAGTGGGTGA |
| Suggested primer 2: | GTCACAATGGACCTGACGG |