| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.114879 |
| Chromosome: | chromosome 12 |
| Location: | 2516031 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506050 | (1 of 1) K14408 - cleavage stimulation factor subunit 3 (CSTF3, RNA14) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGACGCACAGTTTCAAGCCCCGCTCAA |
| Internal bar code: | GCCAATTTCGGCCGTATTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 90.5146 |
| LEAP-Seq n confirming: | 897 |
| LEAP-Seq n nonconfirming: | 94 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTACTGCAGGCGGGACTAC |
| Suggested primer 2: | TCCTCACTCGTCTGCATCAC |