| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.114883 |
| Chromosome: | scaffold 26 |
| Location: | 22643 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre26.g756747 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCAACAACTAAGCGAGCAACGTGAAAA |
| Internal bar code: | CGGCCCACTGTACTCCGGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 400 |
| LEAP-Seq percent confirming: | 99.3051 |
| LEAP-Seq n confirming: | 1572 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTTCAAGCGCTTCACCCT |
| Suggested primer 2: | GCGACACACTGCACTTCAAT |