| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.114901 |
| Chromosome: | chromosome 9 |
| Location: | 39251 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386143 | (1 of 2) 1.14.11.4 - Procollagen-lysine 5-dioxygenase / Procollagen-lysine,2-oxoglutarate 5-dioxygenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGCACAGCCCCACGCCGCCCAGCTCCA |
| Internal bar code: | CACCGGATTATAGTCTTTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 99.8642 |
| LEAP-Seq n confirming: | 2206 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTATGAAGCGCCTGCGTAT |
| Suggested primer 2: | TCCAGCAGATAGTGGCACAG |