| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114904 |
| Chromosome: | chromosome_4 |
| Location: | 3039465 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g225200 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTTATGAAGGCAGGGGCTGCGTCAACGAC |
| Internal bar code: | CTGTCTGTGTGGATGGTGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 16 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCAGGACTGAGCATCTG |
| Suggested primer 2: | GGCTGGCTCACAGAGTTTTC |