| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114932 |
| Chromosome: | chromosome 12 |
| Location: | 2034414 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g510000 | (1 of 2) PF05542 - Protein of unknown function (DUF760) (DUF760) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAGTAAGGTGTGCCTGGGCCGAGAGCAT |
| Internal bar code: | ATCGGCCCGCGTAGGAAGTGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 801 |
| LEAP-Seq percent confirming: | 99.6656 |
| LEAP-Seq n confirming: | 2384 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGCTATGTTTTCCTTGTG |
| Suggested primer 2: | TGTGCTGCCAAGAACGTTAG |