Insertion junction: LMJ.RY0402.114964_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.114964
Chromosome:chromosome 12
Location:1804958
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g512050 (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGATACTGGAAGCGCCCCGGGTCGAAACG
Internal bar code:TCATGGGGCTTTGGGCAGGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:698
LEAP-Seq percent confirming:94.9003
LEAP-Seq n confirming:2475
LEAP-Seq n nonconfirming:133
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTGGAGTTCAAAGTTCGC
Suggested primer 2:AACGACGTGAGCATTTACCC