| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114978 |
| Chromosome: | chromosome 2 |
| Location: | 1657075 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085600 | (1 of 2) IPR005645//IPR029058 - Serine hydrolase FSH // Alpha/Beta hydrolase fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACACCGTTTGCAAGCACAAATACGGCAA |
| Internal bar code: | AGCTACCACGTGCGGGCGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 96.6082 |
| LEAP-Seq n confirming: | 7662 |
| LEAP-Seq n nonconfirming: | 269 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACGGAAAGAGTGCTGGAA |
| Suggested primer 2: | TTCAGGTGAGCAGGTGTGAG |