Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.115011 |
Chromosome: | chromosome_4 |
Location: | 2753875 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g223650 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGAACGCTTCCCACCTCACGGCAGCTAGC |
Internal bar code: | TGTAGCTTGTTTGTTACTCCTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1032 |
LEAP-Seq percent confirming: | 99.7218 |
LEAP-Seq n confirming: | 2868 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGCCCATTCTTCATTTGCT |
Suggested primer 2: | AAGAGCGTCGGCAAAAAGTA |