Insertion junction: LMJ.RY0402.115019_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.115019
Chromosome:chromosome_7
Location:5179511
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g348450 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CCGTCACCCCTACGCTGCGTCACCGGCGGC
Internal bar code:GGCGGGAACAACCGGGTATGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:653
LEAP-Seq percent confirming:95.6853
LEAP-Seq n confirming:754
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACACGTTCCGAATTGCCTAC
Suggested primer 2:GTCTACATACCGCTGCACGA