Insertion junction: LMJ.RY0402.115019_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.115019
Chromosome:chromosome_7
Location:5179519
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g348450 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TTTAATGCAGAAGTTGGCTGTGAGAAGGGG
Internal bar code:CTCATGTCAAGAAGACCATGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1021
LEAP-Seq percent confirming:99.7516
LEAP-Seq n confirming:3213
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACACGTTCCGAATTGCCTAC
Suggested primer 2:GTCTACATACCGCTGCACGA