Insertion junction: LMJ.RY0402.115035_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.115035
Chromosome:chromosome 6
Location:7954469
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g303572 (1 of 8) PF01697 - Glycosyltransferase family 92 (Glyco_transf_92) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATCCAATCTGCCACAGACAGGTGGTGTG
Internal bar code:ACTGACCTGCGAGATTGACGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:206
LEAP-Seq percent confirming:99.7018
LEAP-Seq n confirming:1003
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTTTTCCAGACAGGAGAG
Suggested primer 2:GCCACCTGGTGACGTACTTT