Insertion junction: LMJ.RY0402.115044_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.115044
Chromosome:chromosome 11
Location:3184981
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g479383 (1 of 1) IPR000104//IPR013122 - Antifreeze protein, type I // Polycystin cation channel, PKD1/PKD2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGCTGCCGTACGACGCGGCCACCCCGGCG
Internal bar code:ATTCCGTCTCGTCCCGATTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:188
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCAACAGCTCGACTGAAACG
Suggested primer 2:CAGTGGATACCATGTGGCTG