| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.115082 |
| Chromosome: | chromosome 2 |
| Location: | 4567268 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100850 | (1 of 1) IPR000104//IPR005828//IPR011701//IPR020846 - Antifreeze protein, type I // Major facilitator, sugar transporter-like // Major facilitator superfamily // Major facilitator superfamily domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACCAGCCTCANCAGCAGTAGCAACAGC |
| Internal bar code: | CGGACTACGAAGCGAACCCGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5 |
| LEAP-Seq percent confirming: | 8.69565 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTACGCCTGTCTCACATT |
| Suggested primer 2: | CCCAAACACACATGCAACTC |