| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.115083 |
| Chromosome: | chromosome 6 |
| Location: | 8891630 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311000 | FBT2 | (1 of 4) PTHR31585:SF0 - FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTIC; putative folate/pteridine transporter 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAAAGACTGGAAACTGAAATCTTGAGAC |
| Internal bar code: | TCCCGGGATTTCGTCCCGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1009 |
| LEAP-Seq percent confirming: | 99.8174 |
| LEAP-Seq n confirming: | 2733 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATTGCTCTTTTTGTTCA |
| Suggested primer 2: | GCCAAACACGTAGGTCCTGT |